Hanan Moawia Ibrahim
Ministry of Higher Education & Scientific Research, Sudan
Title: Isolation and characterization of thermophilic Streptomyces sp. with potential production of actinokinase
Biography
Biography: Hanan Moawia Ibrahim
Abstract
The study aimed to produce a potent fibrinolytic enzyme actinokinase from local isolate in batch fermentation, culture of identified thermophilic Streptomyces spp. grown on glucose yeast extract peptone medium pH of 8.0. The hemolytic activity of the crude enzyme and time spend for complete lysis was tested using blood agar media and test tubes containing clotted blood. The in vivo clot lysis of the crude enzyme was found to be faster (20 minutes) compared to the other commercial fibrinolytic enzyme (90 minutes). The enzyme was stable at a broad range of pH ranging from 5 to 9. The thrombolytic potential of this particular isolate indicated that it could extract a promising actinokinase with potent activity.